primer select module of the lasergene program (DNASTAR)
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primer select module of the lasergene program
Primer Select Module Of The Lasergene Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+select+module+of+the+lasergene+program/primerselect+dnastar/pmc08400278-271-16-18
Average 90 stars, based on 1 article reviews
Primer Select Module Of The Lasergene Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+select+module+of+the+lasergene+program/primerselect+dnastar/pmc08400278-271-16-18
Average 90 stars, based on 1 article reviews
primer select module of the lasergene program - by Bioz Stars,
2026-10
90/100 stars
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Multiplex Assay:Article Title: Detection of aacA-aphD, qacEδ1, marA, floR, and tetA genes from multidrug-resistant bacteria: Comparative analysis of real-time multiplex PCR assays using EvaGreen(®) and SYBR(®) Green I dyes. Article Snippet: We have developed multiplex real-time PCR assays that utilize DNA-intercalating dyes, SYBR Green I (SG) and EvaGreen (EG), with two primer sets (set 11⁄4 qacEd1, tetA and aacA-aphD; set 21⁄4 tetA,marA, and floR) to simultaneously amplify the qacEd1, tetA, aacA-aphD,marA, and floR genes.. Validity of the multiplex PCR assays was confirmed by testing 83 bacterial isolates, including Staphylococcus aureus (28 isolates), Enterococcus spp. (17 isolates), Salmonella enterica serovar Typhimurium (8 isolates), Citrobacter spp. (9 isolates), Escherichia coli (14 isolates) and Aeromonas veronii (7 isolates), and performing sequence analysis of representative PCR products.. Agarose gel analysis revealed the presence of correct size PCR products, and the differences in their thermal melting (Tm) curves were used to distinguish various PCR products. Real-time Polymerase Chain Reaction:Article Title: Detection of aacA-aphD, qacEδ1, marA, floR, and tetA genes from multidrug-resistant bacteria: Comparative analysis of real-time multiplex PCR assays using EvaGreen(®) and SYBR(®) Green I dyes. Article Snippet: We have developed multiplex real-time PCR assays that utilize DNA-intercalating dyes, SYBR Green I (SG) and EvaGreen (EG), with two primer sets (set 11⁄4 qacEd1, tetA and aacA-aphD; set 21⁄4 tetA,marA, and floR) to simultaneously amplify the qacEd1, tetA, aacA-aphD,marA, and floR genes.. Validity of the multiplex PCR assays was confirmed by testing 83 bacterial isolates, including Staphylococcus aureus (28 isolates), Enterococcus spp. (17 isolates), Salmonella enterica serovar Typhimurium (8 isolates), Citrobacter spp. (9 isolates), Escherichia coli (14 isolates) and Aeromonas veronii (7 isolates), and performing sequence analysis of representative PCR products.. Agarose gel analysis revealed the presence of correct size PCR products, and the differences in their thermal melting (Tm) curves were used to distinguish various PCR products. |